A simple, rapid, economical, precise and accurate reverse phase high performance liquid chromatography (RP-HPLC) method for estimation of doxophylline and acebrophylline in bulk and tablet dosage form had been developed. The separation was attained by Column Hypersil BDS C18, 150 × 4.6 mm, 5 µ and 10mM dipotassium hydrogen phosphate + 0.1% TEA (pH 5.0): Acetonitrile (50:50 v/v) as mobile phase at a flow rate 1.0 ml per minute. Detection was carried out of wavelength of 271 nm retention time of doxophylline were found to be 4.293 min and acebrophylline were found to be 5.526 min to be respectively. The method has been validated for linearity, accuracy and precision. Linearity observed for range of 16.0 to 56.0 μg/ml for doxophylline and 4.0 to 14.0 μg/ml for acebrophylline. The percentage recovery obtained for doxophylline were found to be in range 99.09 to 100.05 and acebrophylline were found to be in range 99.06 to 100.00, respectively. Development method was found to be accurate, precise and rapid for estimation of doxophylline and acebrophylline in bulk and combined dosage form.
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